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Image Search Results
Journal: Molecular and Cellular Biology
Article Title: Molecular Mechanisms of Fenofibrate-Induced Metabolic Catastrophe and Glioblastoma Cell Death
doi: 10.1128/MCB.00562-14
Figure Lengend Snippet: Effect of intracranially delivered FF on glioblastoma tumor growth. (A) Effect of FF on the clonogenic growth of U-87MG human glioblastoma cells stably expressing the luciferase reporter (U-87MG–luc), which were used in intracranial glioblastoma model. (B) Measurement of intracranial tumor growth after intracranial injection of FF. U-87MG–luc cells (1 × 105) were implanted into the brains of immunodeficient mice (Foxn1nu; Harlan Laboratories). Tumor-bearing mice were subsequently treated with 5 μl of DMSO (control) or 5 μl of 1 mM FF in DMSO by injection at the same place where the tumor cells were implanted. Two weeks later, bioluminescence imaging was done with the Xenogen IVIS 200 system. Tumor size is expressed as radiance (photons/s/cm2/sr) and was quantified with the Living Image 4.1 software according to the manufacturer's recommendations (Xenogen). Data in panel C represent the average radiance from 10 mice ± the standard deviation calculated from two separate experiments. An asterisk indicates a value statistically significantly different from that of DMSO-treated control mice.
Article Snippet:
Techniques: Stable Transfection, Expressing, Luciferase, Injection, Imaging, Software, Standard Deviation
Journal: Molecular and Cellular Biology
Article Title: Molecular Mechanisms of Fenofibrate-Induced Metabolic Catastrophe and Glioblastoma Cell Death
doi: 10.1128/MCB.00562-14
Figure Lengend Snippet: Metabolic and survival responses of glial cells to FF. (A to D) Effects of FF on OCR in monolayer cultures of NHA, primary human GBM cells, and two human glioblastoma cell lines, LN-229 and U-87MG. OCR values were registered in serum-free XF assay medium following the sequential injection of FF (50 μM), oligomycin (0.3 μM), FCCP (0.5 μM), and rotenone (0.3 μM). Control cells were injected with DMSO in XF medium. The results are average OCR values from three experiments performed in triplicate (n = 9) ± the standard deviations. An asterisk indicates a statistically significant difference between control and FF-treated samples (percent decrease in OCR after FF injection), two asterisks indicate a statistically significant difference between percent decreases in OCR after oligomycin injection, three asterisks indicate a statistically significant difference between percent increases in OCR after FCCP injection, and four asterisks indicate a statistically significant difference between percent decreases in OCR after rotenone injection. (E) Survival of LN-229, U-87MG, and primary GBM cells and NHA evaluated with the flow cytometry-based 7-AAD assay. Cells were cultured in the corresponding growth-promoting media in the presence or absence of 25 or 50 μM FF for 72 h. The data are average values from three experiments performed in triplicate (n = 9). An asterisk indicates a statistically significant difference from the corresponding control, two asterisks indicate a statistically significant difference between NHA and LN-229, three asterisks indicate a statistically significant difference between NHA and U-87MG, and four asterisks indicate a statistically significant difference between NHA and GBM. (F) GFAP/DAPI-labeled primary gliosphere that was allowed to adhere and spread following 5 weeks of continuous growth in suspension. Note that all tumor cells are positive for the glial marker GFAP.
Article Snippet:
Techniques: XF Assay, Injection, Flow Cytometry, Cell Culture, Labeling, Marker